Optimization Process of the Pepsin-Solubilized Collagen from Lizardfish (Saurida tumbil Bloch, 1795) Skins by-Product
DOI:
https://doi.org/10.22437/ifstj.v7i1.30797Keywords:
pepsin-aided extraction, optimization process, collagen yield, Marine sourceAbstract
By-products from the marine fish processing are rich in organic compounds that can be converted into value-added products like collagen, and it is thought as an ideal candidate polymer for such research and medical applications. The lizardfish (Saurida tumbil Bloch, 1795) skin collagen had been investigated by our previous work, but an effective extraction method is needed to increase the yield of collagen. The purpose of this study was to optimize the method used to extract pepsin-solubilized collagen (PSC) from lizardfish skin. We employed an approach of one factor at a time (OFAT), along with response surface methodology (RSM) utilizing a central composite design (CCD), to attain the highest possible yield of the extracted collagen. Additionally, its properties were also assessed comparatively. The suggested optimal conditions for extraction were a pepsin concentration of 1.87%, a liquid-solid ratio of 24.90 mL/g, and a hydrolysis period of 38.09 h. Using these conditions resulted in a PSC yield of 21.82 g/100g, which closely matched the predicted collagen value.
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Fatiroi, N.S.; Jaziri, A.A.; Shapawi, R.; Mokhtar, R.A.M.; Noordin, W.N.M.; Huda, N. Biochemical and Microstructural Characteristics of Collagen Biopolymer from Unicornfish (Naso Reticulatus Randall, 2001) Bone Prepared with Various Acid Types. Polymers (Basel) 2023, 15, doi:10.3390/polym15041054.
Ahmed, R.; Haq, M.; Chun, B.S. Characterization of Marine Derived Collagen Extracted from the By-Products of Bigeye Tuna (Thunnus Obesus). Int J Biol Macromol 2019, 135, 668–676, doi:10.1016/j.ijbiomac.2019.05.213.
Wang, H.; Liang, Y.; Wang, H.; Zhang, H.; Wang, M.; Liu, L. Physical-Chemical Properties of Collagens from Skin, Scale, and Bone of Grass Carp (Ctenopharyngodon Idellus). Journal of Aquatic Food Product Technology 2014, 23, 264–277, doi:10.1080/10498850.2012.713450.
Cao, J.; Duan, Q.; Liu, X.; Shen, X.; Li, C. Extraction and Physicochemical Characterization of Pepsin Soluble Collagens from Golden Pompano (Trachinotus Blochii) Skin and Bone. Journal of Aquatic Food Product Technology 2019, 28, 837–847, doi:10.1080/10498850.2019.1652216.
Chen, S.; Chen, H.; Xie, Q.; Hong, B.; Chen, J.; Hua, F.; Bai, K.; He, J.; Yi, R.; Wu, H. Rapid Isolation of High Purity Pepsin-Soluble Type I Collagen from Scales of Red Drum Fish (Sciaenops Ocellatus). Food Hydrocoll 2016, 52, 468–477, doi:10.1016/j.foodhyd.2015.07.027.
Matarsim, N.N.; Jaziri, A.A.; Shapawi, R.; Mokhtar, R.A.M.; Noordin, W.N.M.; Huda, N. Type I Collagen from the Skin of Barracuda (Sphyraena Sp.) Prepared with Different Organic Acids: Biochemical, Microstructural and Functional Properties. J Funct Biomater 2023, 14, doi:10.3390/jfb14020087.
Oslan, S.N.H.; Shapawi, R.; Mokhtar, R.A.M.; Noordin, W.N.M.; Huda, N. Characterization of Acid- and Pepsin-Soluble Collagen Extracted from the Skin of Purple-Spotted Bigeye Snapper. Gels 2022, 8, doi:10.3390/gels8100665.
Johny, L.C.; Kudre, T.G.; Suresh, P. V. Acid and Pepsin Soluble Collagens from Skin By-Product of Red-Bellied Pacu (Piaractus Brachypomus): Extraction and Comparative Characterizations Towards Finding Substitute to Bovine and Porcine Collagen. Journal of Aquatic Food Product Technology 2021, 30, 364–376, doi:10.1080/10498850.2021.1884374.
Prihanto, A.A.; Jaziri, A.A.; Pratomo, M.D.; Putri, S.E.; Fajriati, C.; Nurdiani, R.; Firdaus, M. Characteristics of Collagen from Parrotfish (Chlorurus Sordidus), Tiger Grouper (Epinephelus Fuscoguttatus) and Pink Ear Emperor (Lethrinus Lentjan): Effect of Acetic Acid Concentration and Extraction Time. Online J Biol Sci 2022, 22, 26–35, doi:10.3844/ojbsci.2022.26.35.
Han, Q.Y.; Koyama, T.; Watabe, S.; Nagashima, Y.; Ishizaki, S. Isolation and Characterization of Collagen and Collagen Peptides with Hyaluronidase Inhibition Activity Derived from the Skin of Marlin (Istiophoridae). Molecules 2023, 28, doi:10.3390/molecules28020889.
Ramle, S.Z.; Oslan, S.N.H.; Shapawi, R.; Mokhtar, R.A.M.; Noordin, W.N.M.; Huda, N. Biochemical Characteristics of Acid-Soluble Collagen from Food Processing By-Products of Needlefish Skin (Tylosurus Acus Melanotus). Applied Sciences (Switzerland) 2022, 12, doi:10.3390/app122412695.
Atef, M.; Ojagh, S.M.; Latifi, A.M.; Esmaeili, M.; Udenigwe, C.C. Biochemical and Structural Characterization of Sturgeon Fish Skin Collagen (Huso Huso). J Food Biochem 2020, 44, doi:10.1111/jfbc.13256.
Seixas, M.J.; Martins, E.; Reis, R.L.; Silva, T.H. Extraction and Characterization of Collagen from Elasmobranch Byproducts for Potential Biomaterial Use. Mar Drugs 2020, 18, doi:10.3390/md18120617.
Jaziri, A.A.; Shapawi, R.; Mokhtar, R.A.M.; Noordin, W.N.Md.; Huda, N. Extraction and Characterization of Type I Collagen from Parrotfish (Scarus Sordidus Forsskål, 1775) Scale Solubilized with the Aid of Acetic Acid and Pepsin. Int J Biomater 2023, 2023, 1–10, doi:10.1155/2023/7312447.
Chuaychan, S.; Benjakul, S.; Kishimura, H. Characteristics of Acid- and Pepsin-Soluble Collagens from Scale of Seabass (Lates Calcarifer). LWT 2015, 63, 71–76, doi:10.1016/j.lwt.2015.03.002.
Elbialy, Z.I.; Atiba, A.; Abdelnaby, A.; Al-Hawary, I.I.; Elsheshtawy, A.; El-Serehy, H.A.; Abdel-Daim, M.M.; Fadl, S.E.; Assar, D.H. Collagen Extract Obtained from Nile Tilapia (Oreochromis Niloticus L.) Skin Accelerates Wound Healing in Rat Model via up Regulating VEGF, BFGF, and α-SMA Genes Expression. BMC Vet Res 2020, 16, 352, doi:10.1186/s12917-020-02566-2.
Chen, L.; Cheng, G.; Meng, S.; Ding, Y. Collagen Membrane Derived from Fish Scales for Application in Bone Tissue Engineering. Polymers (Basel) 2022, 14, doi:10.3390/polym14132532.
Department of Fisheries Malaysia Fisheries Statistics I; 2023;
Jaziri, A.A.; Shapawi, R.; Mohd Mokhtar, R.A.; Wan, W.N.; Huda, N. Tropical Marine Fish Surimi By-Products: Utilisation and Potential as Functional Food Application. Food Reviews International 2021.
Jaziri, A.A.; Shapawi, R.; Mokhtar, R.A.M.; Noordin, W.N.M.; Huda, N. Chemical Composition of Lizardfish Surimi By-Product: Focus on Macro and Micro-Minerals Contents. Current Research in Nutrition and Food Science 2021, 9, 52–61, doi:10.12944/CRNFSJ.9.1.06.
Jaziri, A.A.; Shapawi, R.; Mokhtar, R.A.M.; Noordin, W.N.M.; Huda, N. Microstructural and Physicochemical Analysis of Collagens from the Skin of Lizardfish (Saurida Tumbil Bloch, 1795) Extracted with Different Organic Acids. Molecules 2022, 27, doi:10.3390/molecules27082452.
Jaziri, A.A.; Shapawi, R.; Mokhtar, R.A.M.; Noordin, W.N.M.; Huda, N. Biochemical and Microstructural Properties of Lizardfish (Saurida Tumbil) Scale Collagen Extracted with Various Organic Acids. Gels 2022, 8, doi:10.3390/gels8050266.
Jaziri, A.A.; Shapawi, R.; Mokhtar, R.A.M.; Noordin, W.N.M.; Huda, N. Biochemical Analysis of Collagens from the Bone of Lizardfish (Saurida Tumbil Bloch, 1795) Extracted with Different Acids. PeerJ 2022, doi:10.7717/peerj.13103.
Jaziri, A.A.; Shapawi, R.; Mokhtar, R.A.M.; Noordin, W.N.M.; Huda, N. Physicochemical and Microstructural Analyses of Pepsin-Soluble Collagens Derived from Lizardfish (Saurida Tumbil Bloch, 1795) Skin, Bone and Scales. Gels 2022, 8, doi:10.3390/gels8080471.
Veeruraj, A.; Arumugam, M.; Balasubramanian, T. Isolation and Characterization of Thermostable Collagen from the Marine Eel-Fish (Evenchelys Macrura). Process Biochemistry 2013, 48, 1592–1602, doi:10.1016/j.procbio.2013.07.011.
Arumugam, G.K.S.; Sharma, D.; Balakrishnan, R.M.; Ettiyappan, J.B.P. Extraction, Optimization and Characterization of Collagen from Sole Fish Skin. Sustain Chem Pharm 2018, 9, 19–26, doi:10.1016/j.scp.2018.04.003.
Blanco, M.; Vázquez, J.A.; Pérez-MartÃn, R.I.; Sotelo, C.G. Collagen Extraction Optimization from the Skin of the Small-Spotted Catshark (S. Canicula) by Response Surface Methodology. Mar Drugs 2019, 17, doi:10.3390/md17010040.
Mohammadi, R.; Mohammadifar, M.A.; Mortazavian, A.M.; Rouhi, M.; Ghasemi, J.B.; Delshadian, Z. Extraction Optimization of Pepsin-Soluble Collagen from Eggshell Membrane by Response Surface Methodology (RSM). Food Chem 2016, 190, 186–193, doi:10.1016/j.foodchem.2015.05.073.
Yu, F.; Zong, C.; Jin, S.; Zheng, J.; Chen, N.; Huang, J.; Chen, Y.; Huang, F.; Yang, Z.; Tang, Y.; et al. Optimization of Extraction Conditions and Characterization of Pepsin-Solubilised Collagen from Skin of Giant Croaker (Nibea Japonica). Mar Drugs 2018, 16, doi:10.3390/md16010029.
Yang, L.; Wu, X.; Feng, W.; Zhao, T.; Zhou, Y.; Li, F.; Zou, Y.; Bai, S.; Wang, W. Optimization of Enzyme-Assisted Extraction and Characterization of Collagen from Chinese Sturgeon (Acipenser Sturio Linnaeus) Skin. Pharmacogn Mag 2013, 9, 32, doi:10.4103/0973-1296.117859.
Hamdan, F.S.; Sarbon, N.M. Isolation and Characterisation of Collagen from Fringescale Sardinella (Sardinella Fimbriata) Waste Materials; 2019; Vol. 26;.
Luo, Q. Bin; Chi, C.F.; Yang, F.; Zhao, Y.Q.; Wang, B. Physicochemical Properties of Acid- and Pepsin-Soluble Collagens from the Cartilage of Siberian Sturgeon. Environmental Science and Pollution Research 2018, 25, 31427–31438, doi:10.1007/s11356-018-3147-z.
Song, W.K.; Liu, D.; Sun, L.L.; Li, B.F.; Hou, H. Physicochemical and Biocompatibility Properties of Type I Collagen from the Skin of Nile Tilapia (Oreochromis Niloticus) for Biomedical Applications. Mar Drugs 2019, 17, doi:10.3390/md17030137.
Tamilmozhi, S.; Veeruraj, A.; Arumugam, M. Isolation and Characterization of Acid and Pepsin-Solubilized Collagen from the Skin of Sailfish (Istiophorus Platypterus). Food Research International 2013, 54, 1499–1505, doi:10.1016/j.foodres.2013.10.002.
Matmaroh, K.; Benjakul, S.; Prodpran, T.; Encarnacion, A.B.; Kishimura, H. Characteristics of Acid Soluble Collagen and Pepsin Soluble Collagen from Scale of Spotted Golden Goatfish (Parupeneus Heptacanthus). Food Chem 2011, 129, 1179–1186, doi:10.1016/j.foodchem.2011.05.099.
Moula Ali, A.M.; Benjakul, S.; Kishimura, H. Molecular Characteristics of Acid and Pepsin Soluble Collagens from the Scales of Golden Carp (Probarbus Jullieni). Emir J Food Agric 2017, 29, 450–457, doi:10.9755/ejfa.2016-09-1316
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