PENGARUH DIET PUASA INTERMITTENT TERHADAP UREUM KREATININ PADA TIKUS PUTIH MODEL DIABETES MELITUS

Ikmal Galih Saputra; Huntari Harahap; Susan Tarawifa; Ahmad Syauqy; Budi Justitia

doi:10.22437/e-sehad.v4i1.30620

Authors

Ikmal Galih Saputra Jambi University
Huntari Harahap Jambi University
Susan Tarawifa Jambi University
Ahmad Syauqy Jambi University
Budi Justitia Jambi University

DOI:

https://doi.org/10.22437/e-sehad.v4i1.30620

Keywords:

PTM screening; , Primary Health Care Center, intermitten fasting, urea and creatinin level, diabetic model

Abstract

ABSTRACT

Diabetes Mellitus (DM) type 2 is a common metabolic disorder characterized by hyperglycemia. The most frequent microvascular complication in diabetes patients is diabetic nephropathy. Dietary management is one of the approaches in DM treatment. Intermittent fasting as a dietary intervention has been shown to improve overall health, weight management, blood pressure, and glomerular filtration rate (GFR). This study aims to investigate the effects of intermittent fasting on urea and creatinine levels in Wistar rats with a diabetes model. This study is a quantitative experimental analysis conducted in vitro in a laboratory setting, using a pretest and post-test group design. There was no difference in urea and creatinine levels before and after the intermittent fasting diet treatment in Wistar white rats. The one-way ANOVA and Kruskal-Wallis tests indicated no significant differences among the groups, as evidenced by the statistical test results with p-values > 0.05 (not significant). There was no difference in urea and creatinine levels before and after the intermittent fasting diet treatment in Wistar white rats.

Keywords: Diabetes mellitus, intermittent fasting, urea, creatinine, white rats

References

1. Utami PR, Fuad K. Gambaran Kadar Hemoglobin Pada Penderita Diabetes Melitus Komplikasi Ginjal. J Kesehat PERINTIS (Perintis’s Heal Journal). 2018;5(1):99–105. https://jurnal.upertis.ac.id/index.php/JKP/article/view/103

2. Dewi NP, Christin V, Handayani KR. Uji Soy-Yamghurt Terhadap Kadar Ureum Dan Kreatinin Kondisi Hiperglikemik. Farmakol J Farm. 2021;18(2).

3. Harahap H, Kusdiyah E, Subhan R. THE EFFECT OF INTERMITTENT FASTING ON TRIGLYCERIDE LEVELS IN THE WISTAR STRAIN WHITE RATS (RATTUS NORVEGICUS) DIABETES MELLITUS MODEL. Jambi Medical Journal: Jurnal Kedokteran dan Kesehatan. 2023 Jul 31;11(3):299-304.

4. Mindikoglu AL, Abdulsada MM, Jain A, Choi JM, Jalal PK, Devaraj S, et al. Intermittent Fasting From Dawn To Sunset For 30 Consecutive Days Is Associated With Anticancer Proteomic Signature And Upregulates Key Regulatory Proteins Of Glucose And Lipid Metabolism, Circadian Clock, DNA Repair, Cytoskeleton Remodeling, Immune System. J Proteomics [Internet]. 2020;217(September 2019):103645. Available from: https://doi.org/10.1016/j.jprot.2020.103645

5. Habas E, Errayes M, Habas E, Farfar KL, Alfitori G, Habas AE, et al. Fasting Ramadan in Chronic Kidney Disease (CKD), Kidney Transplant and Dialysis Patients: Review and Update. Cureus. 2022;14(5). https://pubmed.ncbi.nlm.nih.gov/35755525/

6. Tuaputimain S, Lestari E, Sukeksi A. Perbedaan Kadar Kadar Kreatinin Darah Sebelum Dan Sesudah Aktivitas Fisik. J Labora Med. 2020;4(20):47–51.

7. Juraschek SP, Appel LJ, Anderson CAM, Miller ER. Effect Of A High-Protein Diet On Kidney Function In Healthy Adults: Results From The Omniheart Trial. Am J Kidney Dis. 2013;61(4):547–54.

8. Harpin, D., Simadibrata, C., Mihardja, H., & Barasila, A. (2020). Effect of Electroacupuncture on Urea and Creatinine Levels in the Wistar Sepsis Model. Medical Acupuncture, 32(1), 29-37. https://doi.org/10.1089/acu.2019.1369

9. Rabeh, N., Refaat, O., & Hamada, M. (2023). The Effect of Intermittent Fasting and Caloric Restricted Diet on Diabetic Rats. Journal of Advanced Zoology, 44(S6), 1333-1340. https://doi.org/10.17762/jaz.v44is6.2445

10. Alatawi, K. and Alshubaily, F. (2021). Coconut products alleviate hyperglycaemic, hyperlipidimic and nephropathy indices in streptozotocin-induced diabetic wistar rats. Saudi Journal of Biological Sciences, 28(8), 4224-4231. https://doi.org/10.1016/j.sjbs.2021.06.060

11. Muzaffar, H., Faisal, M., Anwar, H., Hussain, A., Khan, J., Muhammad, F., … & Karrar, E. (2020). Fish protein intake is a novel dietary approach for managing diabetes‐associated complications in diabetic Wistar rat model. Food Science & Nutrition, 9(2), 1017-1024. https://doi.org/10.1002/fsn3.2069

12. Njapndounké, B., Saah, M., Kouam, E., Boungo, G., & Ngoufack, F. (2021). Optimum biscuit from Musa sapientum L. and Vigna unguiculata L. composite flour: effect on pancreatic histology, biochemical and hematological parameters of diabetic rats. Heliyon, 7(9), e07987. https://doi.org/10.1016/j.heliyon.2021.e07987

13. Belkacemi, L. (2013). Intermittent Fasting Modulation of the Diabetic Syndrome in Streptozotocin- Injected Rats: Post-Mortem Investigations. The Open Physiology Journal, 5(1), 1-6. https://doi.org/10.2174/1874360920130702001

14. Yamazaki, D., Konishi, Y., Morikawa, T., Kobara, H., Masaki, T., Hitomi, H., … & Nishiyama, A. (2020). Failure to confirm a sodium–glucose cotransporter 2 inhibitor‐induced hematopoietic effect in non‐diabetic rats with renal anemia. Journal of Diabetes Investigation, 11(4), 834-843. https://doi.org/10.1111/jdi.13205

15. Yanardağ, R., Bolkent, S., Saçan, Ö., & Bulan, Ö. (2002). The effects of chard (Beta vulgaris L. var. cicla) extract on the kidney tissue, serum urea and Creatinine levels of diabetic rats. Phytotherapy Research, 16(8), 758-761. https://doi.org/10.1002/ptr.1041

16. Garg, R., Heinzle, E., & Noor, F. (2017). Hepatocytes of Wistar and Sprague Dawley rats differ significantly in their central metabolism. Journal of Cellular Biochemistry, 119(1), 909-917. https://doi.org/10.1002/jcb.26255

17. Amri, J., Alaee, M., Babaei, R., Salemi, Z., Meshkani, R., Ghazavi, A., … & Salehi, M. (2021). Biochanin‐A has antidiabetic, antihyperlipidemic, antioxidant, and protective effects on diabetic nephropathy via suppression of TGF‐β1 and PAR‐2 genes expression in kidney tissues of STZ‐induced diabetic rats. Biotechnology and Applied Biochemistry, 69(5), 2112-2121. https://doi.org/10.1002/bab.2272

18. White, P., Król, E., Szwengiel, A., Tubacka, M., Szczepankiewicz, D., Staniek, H., … & Krejpcio, Z. (2020). Effects of Bitter Melon and a Chromium Propionate Complex on Symptoms of Insulin Resistance and Type 2 Diabetes in Rat Models. Biological Trace Element Research, 199(3), 1013-1026. https://doi.org/10.1007/s12011-020-02202-y

19. Kaur, N., Kishore, L., & Singh, R. (2017). Attenuation of STZ-induced diabetic nephropathy byCucurbita pepoL. seed extract characterized by GCMS. Journal of Food Biochemistry, 41(6), e12420. https://doi.org/10.1111/jfbc.12420

20. Gobinath, D., Madhu, A., Giribhattanavar, P., Srinivasan, K., & Prapulla, S. (2010). Beneficial effect of xylo-oligosaccharides and fructo-oligosaccharides in streptozotocin-induced diabetic rats. British Journal of Nutrition, 104(1), 40-47. https://doi.org/10.1017/s0007114510000243

21. Souza, C., Sattler, J., Assis, A., Rech, A., Perry, M., & Souza, D. (2012). Metabolic Effects of Sulforaphane Oral Treatment in Streptozotocin-Diabetic Rats. Journal of Medicinal Food, 15(9), 795-801. https://doi.org/10.1089/jmf.2012.0016

22. Nurmasitoh, T., Utami, S., Kusumawardani, E., Najmuddin, A., & Fidianingsih, I. (2018). Intermittent fasting decreases oxidative stress parameters in Wistar rats (Rattus norvegicus). Zinc Supplementation Improves Heme Biosynthesis in rats Exposed to Lead, 37(1), 31-38. https://doi.org/10.18051/univmed.2018.v37.31-38

23. Morgan, D., Carver, M., & Payne, R. (1977). Plasma creatinine and urea: creatinine ratio in patients with raised plasma urea.. BMJ, 2(6092), 929-932. https://doi.org/10.1136/bmj.2.6092.929

24. Harahap, H., Kusdiyah, E., & Zami, M. (2025). Effect of Intermittent Fasting on Ureum in Hypertension Patients. Jambi Medical Journal Jurnal Kedokteran Dan Kesehatan, 13(1), 98-102. https://doi.org/10.22437/jmj.v13i1.36586

25. Ramadhani, A. and Ardiansyah, S. (2024). Comparison of Fasting Blood Sugar Levels and Ureum Levels In Diabetes Mellitus Patients and Diabetic Nephropathy Patients. Academia Open, 9(1). https://doi.org/10.21070/acopen.9.2024.9294