Investigation of The Antioxidant Activity of Cinnamon Bark Extracted with Different Solvents

Authors

  • Muhammad Afdal
  • Azhar Kasim Faculty of Agriculture Universiti Putra Malaysia
  • Abdul Razak Alimon Faculty of Agriculture, Universiti Putra Malaysia
  • Norhani Abdullah Institute of Tropical Agriculture, Universiti Putra Malaysia

DOI:

https://doi.org/10.22437/jiiip.v26i1.24368

Keywords:

antioxidant , cinnamon stick, DPPH, flavonoid, phenolic

Abstract

The objective of this study was to evaluate the effect of methanol, ethyl acetate, and hexane for the extraction of cinnamon bark (Cinnamomum burmanni) (CB) on the contents of the total phenolic, total flavonoid and its 1,1,-diphonyl-1-picryl-hydrazyl (DPPH) radical scavenging activity. CB samples were ground with grinder machine in one mm sieve and extracted with treatments to determine parameter measured. All CB samples were extracted using different treatments. Parameter measured were total phenolic, total flavonoid and DPPH. CB was ground and extracted by using three treatments, data collected was statistical analysis and followed with Duncant Test, The highest total phenolic content found when CB extracted with methanol with the value of 111.43 mg gallic acids equivalent (GAE)/g DW while the lowest amount detected in hexane with the amount of 57.23 mg GAE/g dry weight (DW). It was significantly different (P<0.05) from those extracted with ethyl acetate and hexane. There was significant (P<0.05) different for the content of flavonoid among all treatment groups. Methanol was the highest solvent and hexane was the lowest with the amount of 286.27 and 64.73 mg rutin equivalent (RE)/g DW respectively. There was significantly different (P<0.05) for scavenging activity among all treatment. Using methanol in extracting CB showed the highest scavenging activity of 69.59 % among all other treatment groups. It could be concluded that Methanol was the best solution in extracting CB

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References

Afdal, M., Kasim, A., Alimon, A., & Abdullah, N. (2012). Some chemical properties of oil palm decanter meal. African Journal of Biotechnology, 11(27). https://doi.org/10.5897/AJB11.2612

Ammar, R. Ben, Bhouri, W., Sghaier, M. Ben, Boubaker, J., Skandrani, I., Neffati, A., Bouhlel, I., Kilani, S., Mariotte, A.-M., Chekir-Ghedira, L., Dijoux-Franca, M.-G., & Ghedira, K. (2009). Antioxidant and free radical-scavenging properties of three flavonoids isolated from the leaves of Rhamnus alaternus L. (Rhamnaceae) : A structure-activity relationship study. Food Chemistry, 116(1), 258–264. https://doi.org/10.1016/j.foodchem.2009.02.043

A.O.A.C. (1990). Official Methods of Analysis (K. Herlich, Ed.; 15th ed.). Association of Official Analytical Chemist Inc.

Azima, F., Muchtadi, D., Zakaria, F., & Priosoeryanto, B. (2004). Kandungan fitokimia dan aktivitas antioksidan ekstrak cassia vera (Cinnamomum burmanii). Stigma, 12(2), 232–236.

Boots, A. W., Haenen, G. R. M. M., & Bast, A. (2008). Health effects of quercetin: From antioxidant to nutraceutical. European Journal of Pharmacology, 585(2–3), 325–337. https://doi.org/10.1016/j.ejphar.2008.03.008

Bors, W., Michel, C., & Stettmaier, K. (2001). Structure-activity relationships governing antioxidant capacities of plant polyphenols. In Methods in enzymology (Vol. 335, pp. 166–180). https://doi.org/10.1016/S0076-6879(01)35241-2

Brettonnet, A., Hewavitarana, A., DeJong, S., & Lanari, M. C. (2010). Phenolic acids composition and antioxidant activity of canola extracts in cooked beef, chicken and pork. Food Chemistry, 121(4), 927–933. https://doi.org/10.1016/j.foodchem.2009.11.021

Cai, Y.-Z., Mei Sun, Jie Xing, Luo, Q., & Corke, H. (2006). Structure–radical scavenging activity relationships of phenolic compounds from traditional Chinese medicinal plants. Life Sciences, 78(25), 2872–2888. https://doi.org/10.1016/j.lfs.2005.11.004

Cotelle, N. (1996). Antioxidant properties of hydroxy-flavones. Free Radical Biology and Medicine, 20(1), 35–43. https://doi.org/10.1016/0891-5849(95)02014-4

Folin, O., & Ciocalteu, V. (1927). On Tyrosine and Tryptophane Determinations in Proteins. Journal of Biological Chemistry, 73(2), 627–650. https://doi.org/10.1016/S0021-9258(18)84277-6

Hall, C. I. I. (2001). Sources of natural antioxidants: oilseeds, nuts, cereals, legumes, animal products and microbial sources. Antioxidants in Food Practical Applications, 159–209. https://eurekamag.com/research/003/563/003563798.php

Hamilton, R. J. (Richard J., & Allen, J. C. (1994). Rancidity in foods / edited by J.C. Allen and R.J. Hamilton. In Rancidity in foods (3rd ed). Blackie Academic & Professional.

Hariana, A. (2008). Tumbuhan Obat dan Khasiatnya Seri 2. Penebar Swadaya.

Hasanah, M., Nuryani, Y., Djisbar, A., Mulyono, E., Wikardi, E., & Asman, A. (2003). Indonesian cassia (Indonesian cinnamon) in Cinnamon and Cassia The genus Cinnamomum (P. N. Ravindran, K. Nirmal-Babu, & M. Shylaja, Eds.). CRC Press. https://doi.org/10.1201/9780203590874

Havsteen, B. H. (2002). The biochemistry and medical significance of the flavonoids. Pharmacology & Therapeutics, 96(2–3), 67–202. https://doi.org/10.1016/S0163-7258(02)00298-X

Hendra, R. (2010). Biological Activities of Phaleria macrocarpa (Scheff.) Boerl and the Quantitative Analysis of Flavonoids Present in Bioactive Extracts of the Species. Universiti Putra malaysia.

Khokhar, S., & Magnusdottir, S. G. M. (2002). Total Phenol, Catechin, and Caffeine Contents of Teas Commonly Consumed in the United Kingdom. Journal of Agricultural and Food Chemistry, 50(3), 565–570. https://doi.org/10.1021/jf010153l

Lin, J.-Y., & Tang, C.-Y. (2007). Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chemistry, 101(1), 140–147. https://doi.org/10.1016/j.foodchem.2006.01.014

Middleton, E., Kandaswami, C., & Theoharides, T. C. (2000). The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacological Reviews, 52(4), 673–751.

Mishra, K., Ojha, H., & Chaudhury, N. K. (2012). Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results. Food Chemistry, 130(4), 1036–1043. https://doi.org/10.1016/j.foodchem.2011.07.127

Naczk, M., Pegg, R. B., Zadernowski, R., & Shahidi, F. (2005). Radical scavenging activity of canola hull phenolics. Journal of the American Oil Chemists’ Society, 82(4), 255–260. https://doi.org/10.1007/s11746-005-1064-7

Oliveira, I., Coelho, V., Baltasar, R., Pereira, J. A., & Baptista, P. (2009). Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals. Food and Chemical Toxicology, 47(7), 1507–1511. https://doi.org/10.1016/j.fct.2009.03.042

Pokorný, J. (2001). 1 - Introduction. In J. Pokorny, N. Yanishlieva, & M. Gordon (Eds.), Antioxidants in Food (pp. 1–3). Woodhead Publishing. https://doi.org/https://doi.org/10.1016/9781855736160.1

Razali, N., Mat-Junit, S., Abdul-Muthalib, A. F., Subramaniam, S., & Abdul-Aziz, A. (2012). Effects of various solvents on the extraction of antioxidant phenolics from the leaves, seeds, veins and skins of Tamarindus indica L. Food Chemistry, 131(2), 441–448. https://doi.org/10.1016/j.foodchem.2011.09.001

Ribéreau-Gayon, P., Glories, Y., Maujean, A., & Dubourdieu, D. (2006). Phenolic Compounds. In Handbook of Enology (2nd ed., Vol. 2, pp. 141–203). John Wiley & Sons, Ltd. https://doi.org/10.1002/0470010398.ch6

Rice-Evans, C. A., Miller, N. J., & Paganga, G. (1996). Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine, 20(7), 933–956. https://doi.org/10.1016/0891-5849(95)02227-9

Senanayake, U. M., & Wijesekera, R. O. B. (2003). Chemistry of Cinnamon and Cassia. In P. N. Ravindran, K. Nirmal-Babu, & M. Shylaja (Eds.), Cinnamon and Cassia (1st ed.). CRC Press. https://doi.org/10.1201/9780203590874

Seyoum, A., Asres, K., & El-Fiky, F. K. (2006). Structure–radical scavenging activity relationships of flavonoids. Phytochemistry, 67(18), 2058–2070. https://doi.org/10.1016/j.phytochem.2006.07.002

Shan, B., Cai, Y.-Z., Brooks, J. D., & Corke, H. (2007). Antibacterial Properties and Major Bioactive Components of Cinnamon Stick ( Cinnamomum burmannii ): Activity against Foodborne Pathogenic Bacteria. Journal of Agricultural and Food Chemistry, 55(14), 5484–5490. https://doi.org/10.1021/jf070424d

Slinkard, K., & Singleton, V. L. (1977). Total Phenol Analysis: Automation and Comparison with Manual Methods. American Journal of Enology and Viticulture, 28(1), 49–55. https://doi.org/10.5344/ajev.1977.28.1.49

Soest, P. J. Van. (1990). Use of Detergents in the Analysis of Fibrous Feeds. II. A Rapid Method for the Determination of Fiber and Lignin. Journal of AOAC INTERNATIONAL, 73(4), 491–497. https://doi.org/10.1093/jaoac/73.4.491

Trabelsi, N., Megdiche, W., Ksouri, R., Falleh, H., Oueslati, S., Soumaya, B., Hajlaoui, H., & Abdelly, C. (2010). Solvent effects on phenolic contents and biological activities of the halophyte Limoniastrum monopetalum leaves. LWT - Food Science and Technology, 43(4), 632–639. https://doi.org/10.1016/j.lwt.2009.11.003

Valko, M., Rhodes, C. J., Moncol, J., Izakovic, M., & Mazur, M. (2006). Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biological Interactions, 160(1), 1–40. https://doi.org/10.1016/j.cbi.2005.12.009

Verzelloni, E., Tagliazucchi, D., & Conte, A. (2007). Relationship between the antioxidant properties and the phenolic and flavonoid content in traditional balsamic vinegar. Food Chemistry, 105(2), 564–571. https://doi.org/10.1016/j.foodchem.2007.04.014

Wang, H., & Helliwell, K. (2001). Determination of flavonols in green and black tea leaves and green tea infusions by high-performance liquid chromatography. Food Research International, 34(2–3), 223–227. https://doi.org/10.1016/S0963-9969(00)00156-3

Wang, R., Wang, R., & Yang, B. (2009). Extraction of essential oils from five cinnamon leaves and identification of their volatile compound compositions. Innovative Food Science & Emerging Technologies, 10(2), 289–292. https://doi.org/10.1016/j.ifset.2008.12.002

Williams, R. J., Spencer, J. P. E., & Rice-Evans, C. (2004). Flavonoids: antioxidants or signalling molecules? Free Radical Biology and Medicine, 36(7), 838–849. https://doi.org/10.1016/j.freeradbiomed.2004.01.001

Yanishlieva-Maslarova, N. V. (2001). Inhibiting oxidation. In J. Pokorný, N. Yanishlieva, & M. Gordon (Eds.), Antioxidants in food (1st ed., pp. 22–70). Woodhead Publishing Limited. https://doi.org/10.1533/9781855736160.1.22

Yu, L. (2001). Free Radical Scavenging Properties of Conjugated Linoleic Acids. Journal of Agricultural and Food Chemistry, 49(7), 3452–3456. https://doi.org/10.1021/jf010172v

Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555–559. https://doi.org/10.1016/S0308-8146(98)00102-2

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Published

2023-07-17

How to Cite

Afdal, M., Kasim, A., Alimon, A. R., & Abdullah, N. . (2023). Investigation of The Antioxidant Activity of Cinnamon Bark Extracted with Different Solvents. Jurnal Ilmiah Ilmu-Ilmu Peternakan, 26(1), 68-79. https://doi.org/10.22437/jiiip.v26i1.24368

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Research Report