Kualitas Spermatozoa Post Thawing dari Semen Beku Sapi Perah

Abstract

The purpose of this study was to determine the effect of various thawing temperatures to the sperm quality of FH dairy cow frozen semen. The experimental design used was completely randomized design (CRD) with 5 treatments and 5 replications . The thawing treatments were  30 seconds at 33 ⁰C (P1), 35 ⁰C (P2), 37 ⁰C (P3), 39 ⁰C (P4), 41 ⁰C (P5) . Observed variables were sperm motility, viability and recovery rate . Data were analyzed using analysis of variance . The results showed that different thawing temperatures affect the motility , viability, recovery rate of frozen spermatozoa (P<0.05). Motility and  recovery rate of P4 was higher (P< 0.05)  than P2 , P1 and P5  , but did not differ (P>0.05) with P3 . Viability of P4  was higher (P>0.05)  than P1 and P5  but did not differ (P>0.05) with P2 and P3.  In conclusion , thawing at 39 ⁰c and 37 ⁰C for 30 seconds had best semen quality of FH frozen semen.