Naskah ini versi lama yang diterbitkan pada 2019-01-21. Baca versi terbaru.

KOLONISASI BAKTERI ENDOFIT PADA AKAR TUMBUHAN ANDALEH (Morus macroura Miq.)

DOI:

https://doi.org/10.22437/bs.v4i2.5255

Kata Kunci:

Endophytic Bacteria, Morus macroura Miq, Roots, Isolation

Abstrak

Endophytic bacteria are bacteria living in plant tissues and symbiotic mutualism with their host. The excess production of bioactive substances from endophytic microorganisms among others, microorganisms are easy to grow, have shorter life cycles than plants and can produce large amounts of bioactive compounds. Endophytic bacteria found in plants generally originate from the roots and then spread through xylem tissue. Endophytic bacteria that live within the plant tissue for a certain period and are able to live by forming colonies in plant tissue without harming the host. Endophytic bacteria can be isolated from sterile plant tissue surfaces or extracted from inner plant tissue. The purpose of this study was to determine the type of bacterial isolate of edofit found in the root of Andaleh plant. The method used to isolate endophytic  bacteria from the root plant Andaleh  is streak plate method. Identification of bacteria macroscopically and microscopically. The results obtained were 16 isolates of endophytic bacteria from the roots of Andaleh plant seen from different colony forms. Identify microscopically with Gram staining

Unduhan

Data unduhan belum tersedia.

Referensi

1. Afifah, N., Putri, DH., dan Irdawati. 2018. Isolation and Identification of Endophytic Bacteria from the Andalas Plant Stem (Morus macroura Miq.). Bioscience. Vol 2 (1). 72-75
2. Cho, K. M., Hong, S.Y., Lee, S.M., Kim, Y. H., Kahng, G.G., Lim, Y.P., Kim, H., and Yun, H.D. 2007. Endophytic Bacterial Communities in Ginseng and Their Antifungal Activity Against Pathogens. Microbial. Ecol. 54, 341–351.
3. Compant , S., B. R Eiter, A. S Essitch , J. N Owak, C. C Lement , and E.A Barka. 2010. Endophytic Colonization of 4. Vitis vinifera L. by Plant Growth-promoting Bacterium Burkholderia sp. strain PsJN. Applied and Environmental Microbiology 71 : 1685 – 1693 .
5. Grayston, S. J. S. Wang , C. D. Cambell, and A. C. Edwards. 1998. Selective Influence of Plant Species on Microbial Diversity in the Rhizosphere. Soil Biology & Biochemistry. Vol 30 : 369 – 378
6. Hakim, E.H., Achmad, S.A., Juliawaty, L.D., Makmur, L., Syah, Y.M., Aimi, N., Kitajima,M., Takayama, H., and Ghisalberti, E.L. 2007. Prenylated Flavonoids and Related Compounds of the Indonesian Artocarpus (Moraceae). J. Nat. Med. Vol 60: 161-184.
7. Hartmann, A. M. R Othballer, and M. S Chmid. 2008. Lorenz Hiltner, a Pioneer in Rhizosphere Microbial Ecology and Soil Bacteriology Research. Plant and Soil 312 : 7 – 14
8. Hidayat, E.B. 2000. Anatomi Tumbuhan Berbiji. Bandung: ITB Bandung.
9. Jasmansyah. 2002. Kandungan Kimia Maskot Daerah Sumatera. Http:www.chemistry. net firms. com/berita/berita [20 08 2002].
10. Jawezt, E., Melnick., Adelberg’s., Brooks, G. F., Carrol, K. C., Butel, J. S., Morse, S. A., Mietzner, T. A. 2013. Medical Microbiology 26 th Editon. New York :McGraw-Hill Companies, Inc.
11. Kumala, S., Mangunwardoyo, W., & Arvyna, H. 2008. Fermentasi Goyang dan Diam Isolat Bakteri Endofit Buah Makassar (Brucea javanica L. Merr) dan Uji Aktivitas Antimikrobanya. Prosiding Kongres Ilmiah XVI ISFI. Yogyakarta: Ikatan Sarjana Farmasi Indonesia.
12. Lamb, T. G., D.W. Tonkyn, and D.A. Kluepfel. 1996. Movement of Pseudomonas aureofaciens from the Rhizosphere to Aerial Plant Tissue. Microbiol. 42:1112–1120.
13. Putri, MF., Fifendy M., Putri, DH. 2018. Diversitas Bakteri Endofit pada Daun Muda dan Tua Tumbuhan Andaleh (Morus macroura miq.). Eksakta. Vol. 19 (1).
14. Rahman, M. 1991. Flora dan Fauna Identitas Sumatera Barat. Pemda Tk. I Sumatera Barat.
Reinhold-Hurek B, Maes T, Gemmer S, Van Montagu M, Hurek T. An Edoglucanase is Involved in Infection of Rice Roots by the Not cellulose-metabolizing Endophyte Azoarcus sp. Strain BH72. 2006. Mol Plant Microbe Interact . Vol 19.
15. Simarmata, R., Lekatompessy, S., Sukiman, H. 2007. Isolasi Mikroba Endofitik dari Tanaman Obat Sambung Nyawa (Gynura procumbens) dan Analisis Potensinya sebagai Antimikroba. Berkala Penelitian Hayati, 13, 85-90.
16. Zulkifli, L., Soelistya, D., Jekti, D., Mahrus, N., dan Rasmi, C. 2016. Isolasi Bakteri Endofit dari Sea Grass Yang Tumbuh Di Kawasan Pantai Pulau Lombok dan Potensinya Sebagai Sumber Antimokroba Terhadap Bakteri Patogen. Jurnal Biologi Tropis, Juli-Desember 2016: Volume 16 (2):80-93

##submission.downloads##

Diterbitkan

2019-01-21

Versi

Cara Mengutip

KOLONISASI BAKTERI ENDOFIT PADA AKAR TUMBUHAN ANDALEH (Morus macroura Miq.). (2019). BIO-SITE |Biologi Dan Sains Terapan, 4(2), 61 - 67. https://doi.org/10.22437/bs.v4i2.5255

Terbitan

Bagian

Articles