BIO-SITE |Biologi dan Sains Terapan https://online-journal.unja.ac.id/BST <p style="text-align: justify;"><strong><span id="result_box" class="" lang="en"><span class="">Bio-Site Journal </span></span></strong><span id="result_box" class="" lang="en"><span class="">emerges as a forum for scientific publication of research results (not literature review) in the field of pure biology and applied science.</span></span><span id="result_box" class="" lang="en"> <span class="">This journal is published online twice a year in May and November.</span> <span class="">This journal is managed by Biology Department, Faculty of Science and Technology, Universitas Jambi, Indonesia.</span></span></p> <p style="text-align: justify;">Index: <a href="https://scholar.google.co.id/citations?user=JHY1lS4AAAAJ&amp;hl=en">Google Scholar</a>,<a href="https://search.crossref.org/?q=BIOSITE+%7CBIOLOGI+Sains+Terapan">DOI Crossref,</a>&nbsp;<a title="Garuda Index" href="http://garuda.ristekdikti.go.id/journal/view/8171">IPI Garuda</a>&nbsp;<br> ISSN online: <a title="ISSN Online" href="http://issn.pdii.lipi.go.id/issn.cgi?daftar&amp;1449717227&amp;1&amp;&amp;">2502-6178</a></p> en-US <p><a href="http://creativecommons.org/licenses/by/4.0/" rel="license"><img src="https://i.creativecommons.org/l/by/4.0/88x31.png" alt="Creative Commons License"></a></p> <p>This work is licensed under a <a href="http://creativecommons.org/licenses/by/4.0/" rel="license">Creative Commons Attribution 4.0 International License</a>.</p> <p><strong>Copyright Notice:</strong></p> <p>1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.<br>2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.<br>3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (The Effect of Open Access)</p> biosite.fst@unja.ac.id (Bio-Site) aiy98138@gmail.com (Ashif Irvan Yusuf) Wed, 19 May 2021 17:15:41 +0700 OJS 3.2.1.1 http://blogs.law.harvard.edu/tech/rss 60 Isolasi dan Karakterisasi Bakteri Termofilik Penghasil Inulinase dari Sumber Air Panas https://online-journal.unja.ac.id/BST/article/view/8023 <p>Bakteri termofilik penghasil inulinase merupakan bakteri yang dapat menghasilkan enzim inulinase yang tahan pada suhu tinggi.. Penelitian ini bertujuan untuk mengisolasi bakteri termofilik penghasil inulinase dari sumber air panas Padang Ganting Kabupaten Tanah Datar dan mengetahui karakteristik bakteri penghasil inulinase dari sumber air panas. Prosedur penelitian ini dilakukan beberapa tahap yaitu isolasi bakteri dari sumber air panas dengan menggunakan sampel air. Bakteri yang didapatkan dimurnikan sampai dihasilkan koloni tunggal, dan dilakukan pewarnaan gram dari isolat bakteri. Pada penelitian ini diperoleh empat isolat koloni bakteri termofilik penghasil inulinase. Hasil identifikasi secara morfologi menunjukkan bahwa Isolat koloni 1(IKA 1) berbentuk rizoid, permukaan halus, dan warna coklat. Isolat koloni 2 (IKA 2) berbentuk filamen, permukaan mengkilat dan warna coklat. Isolat koloni 3 (IKA 3) berbentuk filamen, permukaan halus,&nbsp; elevasi datar dan berwarna coklat. Isolat koloni 4 (IKA 4) berbentuk bulat, tepi bergelombang, permukaan halus, elevasi datar dan warna coklat. Hasil identifikasi secara mikroskopis (pewarnaan gram) pada keempat isolat koloni tergolong gram negatif dan sel berbentuk batang (bacillus). Kesimpulan adalah ditemukan 4 isolat koloni bakteri termofilik penghasil inulinase dari sumber air panas Padang Ganting Kabupaten Tanah Datar.</p> <p><em>Keywords; s</em><em>e</em><em>l</em><em>ek</em><em>si, karakterisasi, bakteri termofilik, enz</em><em>im, inulinase</em></p> <p>&nbsp;</p> <p>Inulinase producing thermophilic bacteria is a bacteria that can produce a thermostable inulinase enzyme. This study aimed to isolate the inulinase-producing thermophilic bacteria from hot springs Padang Ganting Kabupaten Tanah Datar and knowing the characteristics of inulinase producing thermophilic bacteria from hot springs Padang Ganting Kabupaten Tanah Datar. The procedure is done several steps of research is the isolation bacteria from hot springs Padang Ganting Kabupaten Tanah Datar using water samples. Bacteria acquired up to purified single colonies, staining gram isolate bacterial. In this research get it&nbsp; five isolates inulinase producing thermophilic bacteria. The results of the morphological identification showed that isolates 1 (IKA 1) a rizoid shaped, surface smooth and color of chocolate. Isolates 2 (IKA 2) filament-shaped, shiny surface and color brown . Isolates 3 (IKA 3) filament-shaped, surfaces smooth, elevation and flat brown. Isolate 4 (IKA 4) is round, wavy edges, smooth surface, flat elevation and brown. The results of identified microscopically (gram stain) in all five isolates were classified as gram negative. The conclusion was found 4 isolates of inulinase-producing thermophilic bacterial colonies from hot springs Padang Ganting Kabupaten Tanah Datar.</p> <p>Keywords: selection, characterization, thermophilic bacteria, enzyme, inulinase</p> Husnin Nahry Yarza, Yuni Ahda, Mades Fifendi Copyright (c) 2021 Husnin Nahry Yarza, Yuni Ahda, Mades Fifendi http://creativecommons.org/licenses/by/4.0 https://online-journal.unja.ac.id/BST/article/view/8023 Wed, 19 May 2021 00:00:00 +0700