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Potensi metabolit sekunder produksi bakteri endofit dari tumbuhan laban (Vitex pubescens Vahl) sebagai antikanker
DOI:
https://doi.org/10.22437/chp.v4i2.7937Keywords:
Vitex pubescens Vahl, bakteri endofit, sitotoksik, sel serviks (HeLa)Abstract
Peran mikroba endofit dalam memproduksi metabolit sekunder dengan kualitas yang hampir sama dengan tanaman aslinya sangat potensial untuk dikembangkan. Penelitian ini bertujuan untuk menyelidiki bakteri endofit yang terdapat didalam jaringan kulit batang Laban (Vitex pubescens Vahl) serta kemampuannya untuk memproduksi metabolit bioaktif yang mempunyai aktivitas sitotoksik. Dalam penelitian ini digunakan teknik inokulasi langsung dengan jalan meletakkan sampel tanaman di atas permukaan Nutrient Agar (NA). Metoda Micro tetrazolium (MTT) digunakan untuk menetapkan aktivitas sitotoksik dari ekstrak etilasetat. Bakteri endofit yang berhasil diisolasi sebanyak 3 isolat yaitu Bakteri-2A, Bakteri -3B dan Bakteri-6C. Berdasarkan hasil penapisan dari 3 isolat tersebut didapatkan dua bakteri yang memiliki aktivitas potensial yang baik terhadap sel serviks (HeLa) yaitu isolat Bakteri – 6C (IC50 = 82.54 µg/mL) dan Bakteri-2A (IC50 = 297.09 µg/mL). Isolat yang menunjukkan aktivitas sitotoksik terbesar adalah isolat Bakteri-6C sehingga dapat disimpulkan bahwa isolat tersebut merupakan isolat yang paling potensial sebagai penghasil senyawa antikanker.
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References
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Simarmata R, Lekatompessy S, Sukiman H. 2007. Isolasi mikroba endofitik dati tanaman obat sambung nyawa (Gymura procumbens) dan analisis potensinya sebagai antimikroba. Berk Penel Hayati 13 : 85-90Strobel GA., and B. Daisy, 2003, Bioprospecting for Microbial Endophytesand Their Natural Products, Microbiol. and Mol. Biology Rev.,67(4):491-502.
Strobel, GA., E. Ford. J. Woapong, JK. Harper, AM. Arif, DM. Grant, PCW. Fung, and K. Chan. 2002. Isopestacin, an isobenzopuranone from Pestalotiopsis microspora, possessing antifungal and antioxidant activities. Pytochemistry. 60: 179-183.
Susanty, Dachriyanus, Yanwirasti, F.S. Wahyuni, H. Fadhli, P.A. Aswan. 2018. Aktivitas Sitotoksik Ekstrak Etil Asetat Daun Tampa Badak (Voacanga foetida (Bl.) K.Schum) pada Kanker Kolon HTB-38. Jurnal Sains Farmasi & Klinis. 5(2): 142-146
Zhao, F., He, E., Wang, L., Liu, K. 2008. Anti-tumor Activities of Andrographolide, a diterpene from Andrographis paniculata, by Inducing Apoptosis and Inhibiting VEGF Level. Journal of Asian Natural Products Research. 10(5):473-479.
Miles CO, Di Menna, ME, Jacobs, SWL., Garthwaite, I., Lane, GA., Prestidge, RA., Marshall, SL., Wilkinson, HH., Schardl, CL., Ball, OJP., Latch, GCM., 1998. Endophytic fungi Indigenous Australasian Grasses Associated with Toxicity of Livestock. Appli and Environ Microbio. American Society for Microbiology. 601 – 6
Anwar, L., Santoni, A.,. Putra, D.P., Efdi, M., 2019. Structure Elucidation of a Pentacyclic Triterpenoid and Phenolic from Stem Bark of Vitex Pubescens Vahl. Journal of Chemical Natural Resources. 1(1):68-74
Arunachalam C, Gayathri P. 2010. Studies on bioprospecting of endophytic bacteria from the medicina plant of Andrographis paniculata for their antimicrobial activity and antibiotic susceptibility pattern. Int J of Curr Pharm. Res. 2 (4) : 63-68.
Beck HC, Hansen AM, Lauritsen FR. 2003. Novel pyrazine metabolites found in polymyxin biosynthesis by Paenibacillus polymyxa. FEMS Microbiol Lett. 220: 67–73.
Bhore SJ, Sathisha G. 2010. Screening of endophytic colonizing bacteria for cytokinin-like compounds: crude cell-free broth of endophytic colonizing bacteria is unsuitable in cucumber cotyledon bioassay. World J. Agric. Sci. 6 (4): 345-352.
Castillo U, Harper JK, Strobel GA, Sears J, Alesi K, Ford E, Lin J, Hunter M, Maranta M, Ge H, Yaver D, Jensen JB, Porter H, Robison R, Millar D, Hess WM, Condron M, Teplow D. 2003. Kakadumycins, novel antibiotics from Streptomyces sp.NRRL 30566, an endophyte of Grevillea pteridifolia. FEMS Microbiol. Lett. 224:180-190.
De Kok, R.P.J. 2008. The genus Vitex L (Lamiaceae) in the flora Malesiana region, excluding New Guinea. Kew Buletin 63:17-40.
Desriani, Kusumawati DE, Rivai A, Hasanah N, Amrinola W, Triratna L, Sukma A. 2013. Potential endophytic bacteria for increasing paddy var rojolele productivity. Int. J. on Adv. Sci., Eng. and Information Tech. 3 (1) : 76-78. Ganapaty, S., dan Vidyadhar, K.N., Phytoconstituents and Biological Activities of Vitex-a Review, Journal of Natural Remedies, 5(2):75-95.
Ghaffari, M.M., Ahmad, F., Samzadeh-Kermani, A. 2012. Biological activity of betulinic acid: A review. Pharmacology & Pharmacy. 3:119-123
Jalgaonwala RE, Mohite BV, Mahajan RT. 2010. Evaluation of endophytes for their antimicrobial activity from indigenous medicinal plants belonging to north maharashtra region india . Int. J. on Pharm and Biomed Res. 1 (5) : 136141.
Kementrian Kesehatan RI. 2015. Infodatin, Pusat Data dan Informasi Kementrian Kesehatan RI. Kementrian Kesehatan. Jakarta.
Luo, X., Luo, W., Lin, C., Zhang, L., Li, Y. 2014. Andrographolide Inhibits Proliferation of Human Lung Cancer Cells and the Related Mechanisms. Int J Clin Exp Med. 7(11):4220-4225
Maksum, R. 2005. Peranan Bioteknologi dan Mikroba Endofit Dalam Perkembangan Obat Herbal. Maj. Ilmu Kefarmasian Indonesia. II(3):113-126
Meena, A. K., Niranjan, U.S., Rao, M.M., Padhi, M.M. dan Babu, R., 2011, A Review of the Important Chemical Constituents and Medicinal Uses of Vitex Genus, Asian Journal of Traditional Medicines, 6(2).
Nurani L.H. 2011. Uji sitotoksisitas dan antiproliferatif fraksi etil asetat ekstrak etanol biji jinten hitam (Nigella sativa, Lour) terhadap sel mieloma. Farmasains. 2(1): 11-21.
Padmalatha, K., Jayaram, K., Raju, N.L., Prasad, M.N.V., Arora, R., 2009, Ethnopharmacological and Biotechnological Significance of Vitex, Bioremediation, Biodiversity and Bioavailability, 3(1):6-14.
Putri, H. 2013. Protokol uji sitotoksik metode MTT, Cancer Chemoprevention Research Center (CCRC). Yogyakarta:Fakultas Farmasi, UGM.
Reiner, K. 2016. Catalase test protocol. In: ACM Microbelibrary - Laboratory protocols. American Society for Microbiology, Washington, USA.
Rahman, Ansori. 1992. Pengantar Teknologi Fermentasi. Bogor : PAU IPB.
Simanjuntak P, Bustanussalam, Otovina DM, Rahayuningsih M, Said EG. 2004. Isolasi dan identifikasi artemisinin dari hasil kultivasi mikroba endofit dari tanaman Artemisia annua. [studi mikroba endofitik tanaman Artemisia spp.]. Majalah Farmasi Indonesia. 15(2):6874.
Sukardirman, Harjotaruno, Widyawaruyanti, A., Sismindari, Zaini, N.C. 2007. Apoptosis Inducing Effect of Andrographolide on TD-47 Human Breast Cancer Cell Line. Afr J Traditional CAM. 4(3):345-351.
Schulz, B.; & Boyle, C. 2006. What Are Endophytes? dalam: Schulz, B.; Boyle, C.; & Sieber, T.N. (Eds.). Soil Biology. Volume 9. Microbial Root Endophytes. Springer-Verlag. Berlin Heidelberg. 2006: 1-13. 8
Shweta, S., Bindu, J. H., Raghu, J., Suma, H. K., Manjunatha, B.L., Kumara, P.M., Ravikanth, G., Nataraja, K.N., Ganeshaiah, K. N., Shaanker, R. U. 2013. Isolation of endophytic bacteria producing the anti-cancer alkaloid camptothecine from Miquelia dentata Bedd. (Icacinaceae). Phytomedicine. 20: 913–917.
Simarmata R, Lekatompessy S, Sukiman H. 2007. Isolasi mikroba endofitik dati tanaman obat sambung nyawa (Gymura procumbens) dan analisis potensinya sebagai antimikroba. Berk Penel Hayati 13 : 85-90Strobel GA., and B. Daisy, 2003, Bioprospecting for Microbial Endophytesand Their Natural Products, Microbiol. and Mol. Biology Rev.,67(4):491-502.
Strobel, GA., E. Ford. J. Woapong, JK. Harper, AM. Arif, DM. Grant, PCW. Fung, and K. Chan. 2002. Isopestacin, an isobenzopuranone from Pestalotiopsis microspora, possessing antifungal and antioxidant activities. Pytochemistry. 60: 179-183.
Susanty, Dachriyanus, Yanwirasti, F.S. Wahyuni, H. Fadhli, P.A. Aswan. 2018. Aktivitas Sitotoksik Ekstrak Etil Asetat Daun Tampa Badak (Voacanga foetida (Bl.) K.Schum) pada Kanker Kolon HTB-38. Jurnal Sains Farmasi & Klinis. 5(2): 142-146
Zhao, F., He, E., Wang, L., Liu, K. 2008. Anti-tumor Activities of Andrographolide, a diterpene from Andrographis paniculata, by Inducing Apoptosis and Inhibiting VEGF Level. Journal of Asian Natural Products Research. 10(5):473-479.
Miles CO, Di Menna, ME, Jacobs, SWL., Garthwaite, I., Lane, GA., Prestidge, RA., Marshall, SL., Wilkinson, HH., Schardl, CL., Ball, OJP., Latch, GCM., 1998. Endophytic fungi Indigenous Australasian Grasses Associated with Toxicity of Livestock. Appli and Environ Microbio. American Society for Microbiology. 601 – 6
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Published
2019-12-31
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- 2019-12-31 (1)
How to Cite
Potensi metabolit sekunder produksi bakteri endofit dari tumbuhan laban (Vitex pubescens Vahl) sebagai antikanker. (2019). Chempublish Journal, 4(2), 71-80. https://doi.org/10.22437/chp.v4i2.7937
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