ANTIBACTERIAL ACTIVITY OF ARECA NUT SOAP FORMULATION AGAINST STAPHYLOCOCCUS AUREUS

Abstract

ABSTRACT
Background: Areca nuts are widely grown in Jambi and areca seeds have antibacterial activity, antioxidant, skin
aging and cosmetics. It has the potential to be developed as antibacterial soap.
Objective: This study was to compare the antibacterial effect of betel nut concentration powder and extract in our
soap formulation against Staphylococcus aureus using the in vitro test.
Method: Soap formulation was made with 3 concentration of areca nut powder in soap, namely 1,5 grams/soap
(soap 1); 2,3 grams/soap (soap 2) and 3 grams/soap (soap 3). also with extract of areca nut 1,2 grams/soap (soap
4). The final weight of soap was obtained 50 grams. Antiseptic soap brand X and Y as a control. Antibacterial
activity test using Staphylococcus aureus ATCC 25923 with the well method.
Results: The diameter of the clear zone produced by each areca nut soap was not much different (p>0,05). The
clear zone diameter of soap 1, 2, 3 and 4 were 24.28 ± 7,95 mm, 23,96 ± 6,41 mm, 23,87 ± 6,14 mm, and 23,08
± 1,52 mm respectively. While, diameter of clear zone in brand X and Y were zero. Conclusion: The betel nut
soap formulation in this study has better antibacterial activity against Staphylococcus aureus ATCC 25923 than
control.
Keywords: soap, areca nut, antibacterial, Staphylococcus aureus
ABSTRAK
Latar Belakang: . Pohon pinang banyak ditanam di Jambi dan biji pinang memiliki aktivitas antibakteri, antioksidan,
anti penuaan dan kosmetik. Biji pinang berpotensi untuk dikembangkan sebagai sabun antibakteri.
Tujuan: Penelitian ini membandingkan efek antibakteri sabun pinang dengan bahan aktif dalam bentuk serbuk
dan eksktrak terhadap Staphylococcus aureus menggunakan uji in vitro.
Metode: Formulasi sabun dibuat dengan 3 kadar serbuk pinang dalam sabun, yaitu 1,5 gram / sabun (sabun 1);
2,3 gram / sabun (sabun 2) dan 3 gram / sabun (sabun 3), serta ekstrak biji pinang 1,2 gram/sabun. Berat akhir
sabun diperoleh 50 gram. Sabun antiseptik merek X dan Y sebagai kontrol. Uji aktivitas antibakteri menggunakan
Staphylococcus aureus ATCC 25923 dengan metode sumur.
Hasil: Diameter zona bening yang dihasilkan masing-masing sabun pinang tidak jauh berbeda (p>0,05). Diameter
zona bening sabun 1, 2 , 3 dan 4 masing-masing adalah 24.28 ± 7,95 mm, 23,96 ± 6,41 mm, 23,87 ± 6,14 mm,
dan 23,08 ± 1,52 mm. Sedangkan diameter zona bening pada merek X dan Y adalah nol. Kesimpulan: Formulasi
sabun pinang pada penelitian ini memiliki aktivitas antibakteri yang lebih baik terhadap Staphylococcus aureus
ATCC 25923 daripada sabun control.
Kata kunci: sabun, pinang, antibakteri, Staphylococcus aureus