PERBANDINGAN EKSPRESI GEN BCL 2 PADA PREEKLAMPSIA RINGAN/BERAT DAN KEHAMILAN NORMAL DI KOTA JAMBI

Authors

  • Juwita Universitas Jambi
  • Susan Tarawifa Universitas Jambi
  • Raihanah Suzan Universitas Jambi

Abstract

ABSTRACT

Background: Preeclampsia is a major cause of maternal and perinatal mortality. Failure of the trophoblast to remodeling the spiral arteries results in placental hypoxia. The hypoxic state will cause placental oxidative stress which can lead to an increase in trophoblast apoptosis through the intrinsic pathway. Bcl-2 acts as an antiapoptotic which is expressed to a lesser extent in the apoptotic state. Objectives: To determine characteristics based on maternal age, gestational age, and parity and to determine the ratio of Bcl 2 gene expression in mild/severe preeclamptic pregnancies to normal pregnancies. Methods: This study is a descriptive analytic study with a case control design. It was conducted at the Biomolecular Laboratory of the Faculty of Health Sciences, Jambi University from June 2020 to September 2020. The number of placenta samples taken was 25 samples of mild/severe preeclampsia pregnancies and 25 normal pregnancy samples taken by purposive sampling method. Observation of the Bcl-2 gene expression was carried out with Real Time Polymerase Chain Reaction and then the data were processed computerized by independent t-test. Results: In this study, based on the characteristics of maternal age, mild/severe preeclampsia and normal pregnancy were dominated by ages 20-35 years. Based on gestational age, mild/severe preeclampsia and normal pregnancy were dominated by gestation at term (37-42 weeks). Based on parity, preeclampsia mild/severe and normal pregnancy are dominated by nulliparous. There was a significant difference (p=0.001) in Bcl-2 gene expression in mild/severe preeclampsia (1.68 ± 5.99) compared to normal pregnancy (2.27 ± 5.17). Conclusions: Bcl-2 gene expression was higher in mild/severe preeclampsia compared to normal pregnancy. Keywords: Apoptosis, Placenta, Preeclampsia, Bcl-2 gene.

 

ABSTRAK

Latar Belakang: Preeklampsia merupakan penyebab utama kematian maternal dan perinatal. Kegagalan trofoblas melakukan remodeling arteri spiralis menyebabkan terjadinya hipoksia plasenta. Keadaan hipoksia akan menyebabkan stress oksidatif plasenta yang memicu terjadinya peningkatan apoptosis trofoblas melalui jalur intrinsik. Bcl-2 berperan sebagai antiapoptosis yang diekspresikan lebih rendah pada keadaan apoptosis. Tujuan: Untuk mengetahui karakteristik berdasarkan usia ibu,usia kehamilan, dan paritas serta mengetahui perbandingan ekspresi gen Bcl 2 pada kehamilan preeklampsia ringan/berat dan kehamilan normal. Metode: Penelitian ini adalah penelitian deskriptif analitik dengan desain case control yang dilakukan di Laboratorium Biomolekular Fakultas Ilmu Kesehatan Universitas Jambi pada Juni-September 2020. Jumlah sampel plasenta adalah 25 sampel kehamilan preeklampsia ringan/berat dan 25 sampel kehamilan normal yang diambil dengan metode purposive sampling. Pengamatan ekspresi gen Bcl-2 dilakukan dengan Real Time Polymerase Chain Reaction dan selanjutnya data diolah secara komputerisasi dengan uji t-independent. Hasil: Berdasarkan karakteristik usia ibu, preeklampsia ringan/berat dan kehamilan normal didominasi oleh  usia 20-35 tahun. Berdasarkan usia kehamilan, preeklampsia ringan/berat dan kehamilan normal didominasi oleh kehamilan aterm (37-42 minggu). Berdasarkan paritas, preeklampsia ringan/berat dan kehamilan normal didominasi oleh nulipara. Terdapat perbedaan bermakna (p=0,001) ekspresi gen Bcl-2 pada preeklampsia ringan/berat (1,68±5,99) dibandingkan dengan kehamilan normal (2,27±5,17). Kesimpulan: Ekspresi gen Bcl-2 lebih tinggi pada preeklampsia ringan/berat dibandingkan dengan kehamilan  normal.

Kata KunciApoptosis, Plasenta, Preeklampsia, Gen Bcl-2.

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Published

2021-11-11