Sitotoksisitas Fraksi Piper porphyrophyllum terhadap Sel Kanker T47D

  • Yus Hargono Cahyaning Yudi Universitas Al-Ghifari Bandung
  • Hartanto Nugroho Universitas Gadjah Mada
Keywords: Piper, Cytotoxicity, MTT Assay

Abstract

Genus  Piper  has  been  using  traditionally  as  medicines. Piper aborescen, P. crocatum, P. Imperiale, P.  longum, and P. methysticum have  ability  to  inhibit breast  cancer  proliferation.  This research goal is to know cytotoxic activity of Piper porphyrophyllum fraction against T47D cancer cell. Extraction it’s leaves use soxhlet with chloroform as solvent then use vacuum liquid chromatography. Cytotoxic activity of Piper porphyrophyllum fraction determined by MTT assay. The  result  showed  that fraction group A has lowest IC50 (36.22  µg/ml).

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References

1. Agusta, A. 1998. Minyak Atsiri Tumbuhan Tropika Indonesia, Penerbit ITB, Bandung. Hal, 34-47.
2. Asmarayani, R. & Purnomo. 2004. Hubungan Kekerabatan Antar Spesies Piper Berdasarkan Sifat Morfologi dan
Minyak Atsiri Daun di Yogyakarta. Biodiversitas. 6 (1): 12-16.
3. Bezerra, D.P., Pessoa, C., de Moraes, M.O., Silveira, E.R., Lima, M.A., Elmiro, F.J. & Costa-Lotufo, L.V. 2005.
Antiproliferative Effects of Two Amides, Piperine and Piplartine from Piper Spesies. J. Z. Naturforsch. 60c: 539-543.
4. Jaspars, M., & Tabudravu, J. N. 2005. Anticancer Activities of Constituents of Kava (Piper methysticum). The South
Pasific Journal of Natural Science. 23: 26-29.
5. Miki, Y., Swensen, J., Eidens, D.S., Futreal, P.A. & Skolnick, H.M. 1994. A Strong Candidate for the Breast and
Ovarian Cancer Susceptibility Gene BRCA1. Journal Science New Series. 7: 266-278.
6. Moeljopawiro, S., Nuringtyas, T.R., Noveriza, R., & Trisilawati, O. 2007. Kajian Bioaktif Anti Kanker 3 Varietas Buah
merah: Identifikasi Fraksi Bioaktif Anti Kanker Payudara dan Kanker Rahim dan Mikrobia Kontaminan pada 3
Varietas Buah Merah (Pandanus conoideus Lamk.), Laporan Hasil Penelitian Kerjasama UGM dengan Sekretariat
Badan Penelitian dan Pengembangan Pertanian, Yogyakarta. Hal 23-35.
7. Orjala, J., Erdelmeier, C.A.J., Wright, A.D., Rali, T. & Sticher, O. 1993. Five New Prenylated- Hydroxybenzoic Acid
Derivatives with Antimicrobial and Moluscicidal Activity from Piper aduncum Leaves. Planta Med. 59: 546-551.
8. Parmar, V., Jain, S.C., Bisht, K.S., Jain, R., Taneja, P., Jha, A., Tyagi, O.D., Prasad, A.K., Wengel, J., Olsen, C.E. &
Boll, P.M. 1997. Phytochemistry of the Genus Piper. Phytochemistry. 46 (4): 597-673.
9. Perry, L. M. & Metzger, J. 1980. Medicinal Plants of East and South East Asia. Attributed Properties and Uses, The
MIT Press, London.
10. Raj, L., Ide, T., Gurkar, A.U., Foley, M., Schenone, M., Li, X., Tolliday, N.J., Golub, T.R., Carr, S.A., Shamji, A.F.,
Stern, A.M., Mandinova, A., Schreiber, S.L. & Lee, S.W. 2011. Selective Killing of Cancer Cell by a Small Molecule
Targeting the Stress Response to ROS. J. Nature. 475: 231-234.
11. Reshmi, S.K., Satya, E & Devi, P.S. 2010. Isolation of Piperdine from Piper nigrum and Its Antiproliferative Activity.
African Journal of Pharmacy and Pharmacology. 4 (8): 562- 573.
12. Sarker, S.D., Latif, Z. & Gray, A.I. 2006. Natural Product Isolation 2nd Edition, Hamana Press, New Jersey. Hal 55-
79.
13. Tsai, I.L., Lee, F.P., Wu, C.C., Duh, C.Y., Ishikawa, T., Chen, J.J., Chen, Y.C., Seki, H. & Chen, I.S. 2005. New
Cytotoxic Cyclobutanoid Amides, a New Furanid Lignan and Anti- Platelet Aggregation Constituents from Piper
aborescens. Planta Med. 71 (6): 535-542.
14. Varmus, H. 2012. Natural Product for Cosmetic and Medicine. Presentasi ilmiah disampaikan di UGM, Yogyakarta.
Hal 7-14.
15. Wicaksono, B.D., Handoko, Y.A., Arung, E.T., Kusuma, I.W., Yulia, D., Pancaputra, A.N. & Sandra, F. 2009.
Antiproliferative Effect of the Methanol Extract of Piper crocatum Ruiz & Pav Leaves on Human Breast (T47D) Cells
in Vitro. Trp. J. Pharm. Res. 8 (4): 345-352.
How to Cite
Yudi, Y. H., & Nugroho, H. (1). Sitotoksisitas Fraksi Piper porphyrophyllum terhadap Sel Kanker T47D. BIOSITE |BIOLOGI Sains Terapan, 2(2). Retrieved from https://online-journal.unja.ac.id/index.php/BST/article/view/3413
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