GAMBARAN HISTOPATOLOGI USUS HALUS DAN HATI PADA PUYUH PETELUR YANG DIBERI RANSUM MENGANDUNG KONSENTRAT PROTEIN BUNGKIL INTI SAWIT
Palm Kernel Meal (PKM), one of by products from palm oil processing industry, has high potency as feedstuffs due to its availability. PKM, however, has combination problems of shell contamination, containing high non starch-polysaccharide (NSP) dominated with mannan molecules, and low protein content which causes the utilization of PKM in poultry diet is very limited (< 3%). On the other hand, mannanoligosaccharides from PKM may be used to inhibit pathogenic bacteria in poultry gut. Therefore, separation of cell wall of PKM to isolate mannan molecules and extraction of its protein compounds was alternative methods to solve the feed problem. The protein extraction increases the protein content at least 3 folds. The aims of the study were to investigate the effect of PKMCP inclusion with and without fortification in ration on liver and small intestine histopathology of layer quails. The PKM (200 g) was blended in hot water and/or 0,05N acetic acid (800 ml) for 20 minutes, and centrifuged to separate the supernatant from extracted PKM. Supernatant from hot water, acetic acid and 1 N NaOH extraction were precipitated with 0.1 N HCl with the following treatments: the first extractions were carried out in water + broken glass (E1); water + broken glass (E2); 0,05 N acetic acid + broken glass (E3); or in 0,05 N acetic acid + broken glass (E4), while the second extraction for all PKM residues was carried out in 1N NaOH technical (E1, E3) and pro-analysis (E2, E4), and precipitated with 0.1N HCl until isoelectric point. The best extraction product (R1) in term of recovery protein was also compared chemically and biologically with PKM (R2), and soybean meal (R3) in completely randomized design with 3 treatments and 5 replications. Parameter measured were histopathology evaluation of liver and small intestine. Score lesion of duodenum, jejunum, ileum and liver of quails received PKMCP were 0.70, 0.64, 0.68 and 1.76, while SBM were 0.82, 0.66, 0.76 and 1.02 respectively. It is concluded that PKMCP did not give negative effect on quails performance observed from score lesion and histopathology evaluation of liver and small intestine.
Keywords: Palm Kernel Meal Concenrate, Liver, small inestine, Quail.