PROLIFERASI KALUS DAN EMBRIOGENESIS SOMATIK JARAK PAGAR (JATROPHA CURCAS L.) DENGAN BERBAGAI KOMBINASI ZPT DAN ASAM AMINO (Callii Proliferation and Somatic Embryogenesis of Physic Nut (Jatropha curcas L.) Various Combination with PGR’s and Amino Acids)

Abstract

The aim of this study was to reveal a combination of plant growth
regulators (PGR’s) and amino acids best callii proliferation and somatic
embryogenesis physic nut. The experiment was arranged in completely
randomized design with combination of plant growth regulators (PGR’s)
and amino acids, i.e. 1 ppm TDZ + 1 ppm 2,4-D + 100 ppm glutamine;
2 ppm BAP + 1 ppm 2,4 - D + 100 ppm glutamine; 1 ppm TDZ + 1 ppm
2,4-D + 100 ppm CH (Casein hydrolyzate); 2 ppm BAP + 1 ppm 2,4-D
+ 100 ppm CH (casein hydrolyzate); 1 ppm TDZ + 1 ppm 2,4-D; 2 ppm
BAP + 1 ppm 2,4-D; Mannitol 3%; proline 5,75 ppm; proline 2.875
ppm. The parameters observed were callii weight, callii diameter, callii
characteristics (colour and structure) and the number of embryogenic
callii. The result showed that some treatment combinations of plant
growth regulators and amino acids can increase the callii weight and
callii diameter. The color of celli was dominated by green and cream
with mostly compact structure. Meanwhile, celli friable structure as in
treatment 1 ppm TDZ + 1 ppm 2,4-D; proline 5.75 ppm and 2.875 ppm.
Key words : casein hydrolyzate, BAP, glutamine, proline, TDZ