ISOLASI, IDENTIFIKASI DAN PEMURNIAN CENDAWAN MIKORIZA ARBUSKULAR (CMA) DARI TANAH BEKAS TAMBANG BATU BARA (Isolation, Identification and Purification of Arbuscular Mycorrhiza Fungi (AMF) from Coal Post Mining Soil)

Abstract

Land of coal post-mining is the critical area that generally can not be
cultivated due to very low levels of fertility of the land, so this land
becomes slighted. One of the alternatives to overcome this problem is
through inoculation with Arbuscular Mycorrhiza Fungi (AMF).
Indigenous AMF (from coal post-mining location) is more potential AMF
developing in that area. Therefore, isolation, identification and purification
steps of AMF spores are required. The objective of this study was to
isolate, identify, and purify of arbuscular mycorrhiza fungi from coal post
mining area. The study had identified that at this soil was found 3 AMF
genuses, i.e. Glomus, Acaulospora, and Gigaspora. On coal post-mining
soil was found 20 strains of AMF (13 strains of Glomus, 3 strains of
Acaulospora, and 1 strain of Gigaspora). In this soil was dominated by
Glomus. Strain’s AMF that was successful isolated from single spore
culture was 4 strains i.e. Glomus sp-3, Glomus sp-6, Glomus sp-15, dan
Glomus sp-16.