Exploration of Phosphate-Solubilizing Rhizobacteria Associated with Liberica Tungkal Composite Coffee (Coffea liberica) in Jambi, Indonesia
DOI:
https://doi.org/10.22437/proca.v2i3.60001Keywords:
biofertilizer, Coffea liberica, phosphate-solubilizing bacteria, Pikovskaya agar, rhizosphere bacteria.Abstract
Phosphorus (P) is an essential macronutrient required for plant growth and productivity; however, most phosphorus in soil occurs in insoluble forms that are unavailable for plant uptake. Phosphate-solubilizing bacteria (PSB) play an important role in improving phosphorus availability by converting insoluble phosphate into soluble forms, thereby enhancing nutrient-use efficiency and reducing dependence on chemical fertilizers. This study aimed to explore phosphate-solubilizing rhizobacteria associated with Liberica Tungkal Composite coffee (Coffea liberica) cultivated in Tanjung Jabung Barat and Tanjung Jabung Timur Regencies, Jambi Province, Indonesia. Rhizosphere bacteria were isolated and screened for their phosphate-solubilizing ability on Pikovskaya agar medium. Phosphate-solubilizing activity was evaluated qualitatively based on the formation of clear halos surrounding bacterial colonies. A total of 30 rhizosphere bacterial isolates were successfully obtained. All isolates formed clear halos on Pikovskaya agar medium, with halo diameters ranging from 0.2 to 1.2 cm, indicating their ability to solubilize insoluble phosphate. The variation in halo diameter suggests differences in phosphate-solubilizing activity among the isolates. These findings demonstrate that the rhizosphere of Liberica Tungkal Composite coffee harbors diverse indigenous phosphate-solubilizing bacteria with potential for further characterization and development as biofertilizers to improve phosphorus availability and support sustainable Liberica coffee cultivation.
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