Phytochemical Screening and Antioxidant Activities of Ethanolic Extracts of Metaxya parkeri (Hook. & Grev.) J.Sm.
DOI:
https://doi.org/10.22437/proca.v2i2.55583Keywords:
Metaxya parkeri, antioxidant, DPPH, phytochemicals, ethanolic extractAbstract
Background: Metaxya parkeri (Hook. & Grev.) J.Sm. is a fern species whose pharmacological potential has not been extensively explored. Objective: This study aimed to screen phytochemical compounds and evaluate the antioxidant activity of ethanolic extracts from the petiole and pinnae of M. parkeri. Methods: Extraction was performed using maceration with 96% ethanol. Qualitative phytochemical screening was conducted to detect alkaloids, flavonoids, saponins, tannins, and terpenoids. Antioxidant activity was determined using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method and expressed as the inhibitory concentration 50 (IC50). Results: Both petiole and pinnae extracts tested positive for alkaloids, tannins, saponins, and flavonoids. The ethanolic extracts exhibited weak antioxidant activity, with IC50 values of 160.54 ± 4.63 ppm for the petiole and 117.81 ± 0.61 ppm for the pinnae, whereas ascorbic acid as the positive control demonstrated strong activity (44.75 ± 0.67 ppm). Conclusion: M. parkeri contains bioactive compounds; however, their concentration in the crude ethanolic extract may be insufficient to produce strong antioxidant activity, suggesting the need for further extraction or purification methods.
References
1. Anggarani MA, Ilmiah M, Mahfudhah N. Literature review of antioxidant activity of several types of onions and its potensial as health supplements. Indonesian Journal of Chemical Science. 2023;12(1):103-111. doi:10.15294/IJCS.V12I1.57689.
2. Chandimali N, Bak SG, Park EH, Lim HJ, Won YS, Kim EK, Park SI, Lee SJ. Free radicals and their impact on health and antioxidant defenses: a review. Cell Death Discovery. 2025;11(1):1-17. doi:10.1038/s41420-024-02278-8.
3. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: impact on human health. Pharmacognosy Reviews. 2010;4(8):118-126. doi:10.4103/0973-7847.70902.
4. Zehiroglu C, Ozturk Sarikaya SB. The importance of antioxidants and place in today’s scientific and technological studies. Journal of Food Science and Technology. 2019;56(11):4757-4774. doi:10.1007/s13197-019-03952-x.
5. Azka S, Azirda M. Diversity and bioprospecting of Pteridophyte in the Indigenous Talang Mamak Tribe, Riau, Indonesia. SABRAO Journal of Breeding and Genetics. 2024;56(5):1845-1857. doi:10.54910/sabrao2024.56.5.9.
6. Nurhasnawati H, Sundu R, Sapri, Supriningrum R, Kuspradini H, Arung ET. Antioxidant activity, total phenolic and flavonoid content of several indigenous species of ferns in East Kalimantan, Indonesia. Biodiversitas Journal of Biological Diversity. 2019;20(2):576-580. doi:10.13057/biodiv/d200238.
7. Yumkham SD, Elangbam M, Nongmaithem R, Naorem PD, Singh PK. Maiden hair ferns (Adiantum L., Pteridaceae-Vittarioideae) of North East India: diversity, phytochemistry and utilization. Genetic Resources and Crop Evolution. 2018;65(4):1269-1280. doi:10.1007/s10722-018-0612-y.
8. Chetia P, Dey D, Puzari M, Dutta Choudhury M. Bioactive compounds and biological activities of Dipteris wallichii. In: Murthy HN, editor. Bioactive Compounds in Bryophytes and Pteridophytes. Cham: Springer; 2022. p. 1-10. doi:10.1007/978-3-030-97415-2_15-1.
9. Cardenas GG, Tuomisto H, Lehtonen S. Newly discovered diversity in the tropical fern genus Metaxya based on morphology and molecular phylogenetic analyses. Kew Bulletin. 2016;71(1):1-27. doi:10.1007/s12225-016-9618-9.
10. Remedios TS, Silva ABB, Luciano JS, Pinangé DSB, Amorim BS. Novos registros e reconhecimento morfológico das espécies de Metaxya C. Presl (Metaxyaceae) na Amazônia brasileira. Hoehnea. 2022;49(6):1-13. doi:10.1590/2236-8906-63-2021.
11. Salazar-Aranda R, Pérez-López LA, López-Arroyo J, Alanís-Garza BA, Waksman de Torres N. Antimicrobial and antioxidant activities of plants from northeast of Mexico. Evidence-Based Complementary and Alternative Medicine. 2011;2011:536139. doi:10.1093/ecam/nep127.
12. Phongpaichit S, Nikom J, Rungjindamai N, Sakayaroj J, Hutadilok-Towatana N, Rukachaisirikul V, Kirtikara K. Biological activities of extracts from endophytic fungi isolated from Garcinia plants. FEMS Immunology and Medical Microbiology. 2007;51(3):517-525. doi:10.1111/j.1574-695X.2007.00331.x.
13. Nazila J, Husnudin UB, Ihsan M, Hariyadi B, Maritsa HU. The diversity of terrestrial ferns in the Customary Forest of Depati Karo Jayo Tuo, Jambi. Al-Hayat: Journal of Biology and Applied Biology. 2024;7(2):177-189. doi:10.21580/ah.v7i2.23967.
14. Pagare S, Bhatia M, Tripathi N, Pagare S, Bansal YK. Secondary metabolites of plants and their role: overview. Current Trends in Biotechnology and Pharmacy. 2015;9(3):293-304.
15. Vetter J. Secondary metabolites of ferns. In: Fernández H, editor. Current Advances in Fern Research. Cham: Springer; 2018. doi:10.1007/978-3-319-75103-0_15.
16. Chikmawati T, Sopyati PD, Miftahudin. Pertumbuhan dan analisis kualitatif tanin, saponin dan flavonoid dari Selaginella plana, S. willdenovii, and S. mayeri pada tiga naungan berbeda. Jurnal Bioslogos. 2013;3(1):1-9.
17. Abedelmaksoud TG, Younis MI, Altemimi AB, Tlay RH, Ali Hassan N. Bioactive compounds of plant-based food: extraction, isolation, identification, characteristics, and emerging applications. Food Science & Nutrition. 2025;13:e70351. doi:10.1002/fsn3.70351.
18. Utami YP, Arruansaratu E, Jumaetri F. Analisis kadar total alkaloid dari beberapa ekstrak daun patikala (Etlingera elatior (Jack) R.M. Smith). Prosiding Seminar Nasional Kefarmasian “Metabolomics in Pharmacy: Peluang dan Tantangan Kefarmasian dalam Penemuan, Pengembangan, dan Evaluasi Mutu Obat Bahan Alam”. Manado; 2022. p. 1-6.
19. Amalia R, Anggarani MA. Analisis kadar fenolik, flavonoid dan aktivitas antioksidan umbi bawang bombai (Allium cepa L.). UNESA Journal of Chemistry. 2022;11(1):34-45.
20. Agustina W, Nurhamidah, Handayani D. Skrining fitokimia dan aktivitas antioksidan beberapa fraksi dari kulit batang jarak (Ricinus communis L.). ALOTROP Jurnal Pendidikan dan Ilmu Kimia. 2017;1(2):117-122.
21. Hanifah NI, Wirasisya DG, Muliani AE, Utami SB, Sunarwidhi AL. Phytochemical screening of decoction and ethanolic extract of Amomum dealbatum Roxb. leaves. Jurnal Biologi Tropis. 2021;21(2):510-518. doi:10.29303/jbt.v21i2.2758.
22. Virtbauer J, Kählig H, Hüfner A, Donath O, Marian B. Chemical and pharmacological investigations of Metaxya rostrata. Zeitschrift für Naturforschung C. 2008;63(7-8):469-475. doi:10.1515/znc-2008-7-801.
23. Kurniawati IF, Sutoyo. Review artikel: potensi bunga tanaman sukun (Artocarpus Altilis [Park. I] Fosberg) sebagai bahan antioksidan alami. UNESA Journal of Chemistry. 2021;10(1):1-11.
24. Faizal A, Taufik I, Rachmani AF, Azar AWP. Short communication: antioxidant and antibacterial properties of tree fern Cyathea contaminans. Biodiversitas. 2020;21(5):2201-2205. doi:10.13057/biodiv/d210548.
25. Dia SPS, Nurjanah, Jacoeb AM. Komposisi kimia dan aktivitas antioksidan akar, kulit batang dan daun lindur. Jurnal Pengolahan Hasil Perikanan Indonesia. 2015;18(2):205-219. doi:10.17844/jphpi.2015.18.2.205.
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