In Vitro Compatibility Evaluation of Trichoderma harzianum, Bacillus subtilis, and Pseudomonas fluorescens on King’s B and PDA–NA Media
DOI:
https://doi.org/10.22437/proca.v2i2.55580Keywords:
Trichoderma harzianum, Bacillus subtilis, Pseudomonas fluorescens, compatibility, biocontrolAbstract
In Vitro Compatibility Evaluation of Trichoderma harzianum, Bacillus subtilis, and Pseudomonas fluorescens on King’s B and PDA-NA Media. Background: Trichoderma harzianum, Bacillus subtilis, and Pseudomonas fluorescens are widely used biological control agents, but their combined use requires compatibility assessment to prevent antagonistic interactions in microbial consortia. Objective: This study evaluated the in vitro compatibility of T. harzianum with B. subtilis and P. fluorescens on King’s B and PDA-NA media. Methods: A dual-culture assay was conducted by observing inhibition zones and measuring the colony diameter of T. harzianum at 24 and 48 hours; percentage inhibition and Cohen’s d effect size were then calculated. Results: On King’s B medium, clear inhibition zones were observed and percentage inhibition reached 57.04% in T. harzianum x B. subtilis and 62.89% in T. harzianum x P. fluorescens at 48 hours, indicating strong antagonism. On PDA-NA medium, no inhibition zones were detected and inhibition values were lower, at 10.96% and 18.40%, respectively. Conclusion: The three microorganisms may be combined under nutrient conditions that do not strongly stimulate antagonistic metabolite production, supporting the development of multi-agent biocontrol formulations.
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