Phytochemical screening of alkaloid compounds on microencapsulation of Moringa oleifera leaf extract

Authors

  • Rita Halim Departemen Gizi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi
  • Raihanah Suzan Departemen Gizi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi
  • Hanina Departemen Mikrobiologi dan Parasitologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi
  • Hasna Dewi Departemen Histologi dan Patologi Anatomi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi
  • Fadliyana Ekawaty Departemen Keperawatan Anak, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi
  • Hafizah Departemen Fisiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi
  • Denok Tri Hardiningsih Departemen Fitofarmaka, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

DOI:

https://doi.org/10.22437/proca.v1i2.50324

Keywords:

Microencapsulation; moringa extract; functional foods; antioxidants; alkaloids

Abstract

Background: Moringa oleifera leaves are rich in bioactive compounds, including alkaloids. Alkaloid compounds are crucial for their pharmacological potential and role as natural antioxidants in improving food quality and stability. Objective: This study aimed to examine the presence of alkaloid compounds in the microencapsulation of Moringa oleifera leaf extract as a strategy to protect active compounds from degradation and retain their nutritional benefits. Methods: The Moringa oleifera leaf extract was formulated into microencapsulation using the freeze-drying method. Phytochemical screening for alkaloid compounds was performed using the Dragendorff, Bouchardat, and Wagner tests. Results: The phytochemical screening results confirmed the presence (positive) of alkaloid compounds in the microcapsule preparation. This positive result was characterized by the formation of an intense orange-red precipitate (Dragendorff), a reddish-brown precipitate (Bouchardat), and a brown precipitate (Wagner). Conclusion: The microencapsulation of Moringa oleifera leaf extract successfully presented positively detected alkaloid compounds. The retention of alkaloids as antioxidants in these microcapsules provides a strong foundation for their development as supplements or fortification ingredients in food products to enhance nutritional value.

Author Biographies

  • Rita Halim, Departemen Gizi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

    Departemen Gizi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

  • Raihanah Suzan, Departemen Gizi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

    Departemen Gizi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

  • Hanina, Departemen Mikrobiologi dan Parasitologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

    Departemen Mikrobiologi dan Parasitologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

  • Hasna Dewi, Departemen Histologi dan Patologi Anatomi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

    Departemen Histologi dan Patologi Anatomi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

  • Fadliyana Ekawaty, Departemen Keperawatan Anak, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

    Departemen Keperawatan Anak, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

  • Hafizah, Departemen Fisiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

    Departemen Fisiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

  • Denok Tri Hardiningsih, Departemen Fitofarmaka, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

    Departemen Fitofarmaka, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

References

[1] Ahmad N, et al. Bioactive compounds in Moringa oleifera: a comprehensive review. J Food Biochem. 2023;47(3):e13987. Available from: https://doi.org/10.1111/jfbc.13987

[2] Farooq F, et al. Medicinal properties of Moringa oleifera: An overview of promising healer. J Med Plants Res. 2012;6(27):4368–74. Available from: https://doi.org/10.5897/JMPR12.279

[3] Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. Scientific World J. 2013 Feb 2;2013:162750. Available from: https://doi.org/10.1155/2013/162750

[4] Alia F, et al. The potency of Moringa oleifera lam. As natural antioxidant and its health benefits. Pharm Sci Asia. 2022;49(1):1–10. Available from: https://doi.org/10.29037/psa.v49i1.791

[5] Silva F, et al. Stability of bioactive compounds in food processing: a review. Food Chem. 2024;380:132123. Available from: https://doi.org/10.1016/j.foodchem.2023.132123

[6] Lee JH, et al. Microencapsulation of plant extracts for food applications: recent trends and future perspectives. Food Hydrocolloids. 2025;130:107789. Available from: https://doi.org/10.1016/j.foodhyd.2024.107789

[7] Abramović B, et al. Impact of freeze- and spray-drying microencapsulation techniques on β-glucan powder biological activity: A comparative study. Foods. 2022;11(15):2267. Available from: https://doi.org/10.3390/foods11152267

[8] Szumilo M, et al. Preparation and evaluation of surface solid dispersion of Moringa oleifera leaf extract using freeze-drying method. J Res Pharm. 2025;27(6):2330-41. Available from: https://doi.org/10.1016/j.jrp.2024.08.010

[9] Kadir MA, et al. Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from Ephedra intermedia indigenous to Balochistan. Scientific World J. 2017 Mar 13;2017:3149681. Available from: https://doi.org/10.1155/2017/3149681

[10] Ahmad Nejhad M, et al. Integrated in vitro and in silico evaluation of the antimicrobial and cytotoxic potential of Calotropis procera leaf ethanolic extract: From GC-MS profiling to molecular docking and dynamics. Int J Mol Sci. 2025;26(21):10574. Available from: https://doi.org/10.3390/ijms262110574

[11] Zhang Y, et al. Advances in microencapsulation technology for food bioactive compounds: a review. Trends Food Sci Technol. 2024;130:45-60. Available from: https://doi.org/10.1016/j.tifs.2023.10.009

[12] Putri DA, et al. Development of functional tempe enriched with plant extracts: a review. J Food Sci Technol. 2023;60(5):1234-1245. Available from: https://doi.org/10.1007/s13197-023-05763-7

[13] Gopalakrishnan L, et al. Moringa oleifera: A review on nutritive importance and its medicinal application. Food Sci Hum Wellness. 2016;5(2):49-56. Available from: https://doi.org/10.1016/j.fshw.2016.04.001

[14] Hassan L, et al. Alkaloid bioactive from Annona muricata (Soursop) and its pharmacological benefits. Int J Adv Res. 2022;10(12):803–12. Available from: https://doi.org/10.21474/IJAR01/16024

[15] Nugroho R, et al. Stabilitas antioksidan ekstrak etanol daun kelor (Moringa oleifera Lam) terenkapsulasi dalam maltodekstrin dengan metode spray drying. J Ilm Farm. 2022;18(2):83–91. Available from: https://doi.org/10.20885/jif.v18i2.5539

[16] Madiyanti, et al. Potensi antioksidan dari ekstrak daun kelor (Moringa oleifera Lam.) pada berbagai jenis pelarut. J Trop Pharm Chem. 2023;6(2):167–75. Available from: https://doi.org/10.31539/jtpc.v6i2.4633

[17] Hardiningsih DT, Suhesti TS, Novrial D, Arjadi F. Studi effektivitas nanoemulsi purwoceng pada perbaikan fungsi reproduksi tikus putih jantan pasca induksi sleep deprivation: Study of the Effectiveness of Purwoceng Ethanol Extract Nanoemulsion on Improvement of the Reproductive Function of Male White Rats Post Sleep Deprivation Induction. j. tumbuh. obat. Indonesia. 2023;16(1):30-4. https://doi.org/10.31002/jtoi.v16i1.594

[18] Sarubbo LA, et al. Bioactive compound encapsulation: Characteristics, applications in food systems, and implications for human health. Int J Mol Sci. 2024;25(22):12320. Available from: https://doi.org/10.3390/ijms252212320

[19] Wang Y, et al. Enhancing the stability of Ginkgo biloba extract by microencapsulation using spray drying. Food Sci Technol. 2023;43:e45021. Available from: https://doi.org/10.1590/fst.45021

[20] Yusuf R, et al. Aplikasi teknologi enkapsulasi untuk meningkatkan stabilitas bahan bioaktif: Tinjauan. J Teknol Pertan. 2022;23(2):101–12. Available from: https://doi.org/10.21776/ub.jtp.2022.023.02.3

[21] Rasool S, et al. Microencapsulation: A novel approach for functional food development. J Food Sci Technol. 2024;61(1):1–14. https://doi.org/10.1007/s13197-023-05952-4

[22] Prieto C, et al. Microencapsulation technology for food and nutraceutical applications. Food Rev Int. 2022;38(6):638–60. https://doi.org/10.1080/87559129.2021.1895655

[23] Sudaryati NLG, Utomo B, Adnyana IMDM, et al. Phytochemical profiling, dengue antiviral properties, and cytotoxicity of novel Baper tea polyherbal infusion: Insights from in silico and in vitro studies. Salud, Ciencia y Tecnología. 2025;5:1791. https://doi.org/10.56294/saludcyt20251791

[24] Utami T, et al. Optimasi metode skrining fitokimia untuk senyawa alkaloid pada ekstrak daun kemangi (Ocimum basilicum L.). J Sains Farm Klin. 2023;10(3):201–8. Available from: https://doi.org/10.25077/jsfk.10.3.201-208.2023

[25] Kim T, Park S. Dragendorff reagent: A historical perspective and modern applications in natural product chemistry. Chem Pharm Bull. 2025;73(1):1-10. Available from: https://doi.org/10.1248/cpb.c24-00627

[26] Chen F, Liu G. Optimization of freeze-drying parameters for sensitive bioactive compounds. Food Res Int. 2023;164:112100. https://doi.org/10.1016/j.foodres.2022.112100

[27] Brown K, White J. Quantitative analysis of phenolic compounds in food matrices using HPLC. J Chromatogr A. 2023;1700:463870. https://doi.org/10.1016/j.chroma.2023.463870

[28] Garcia L, Rodriguez M. The role of microencapsulation in enhancing the bioavailability of plant extracts. Nutr Rev. 2024;82(3):210-225. https://doi.org/10.1093/nutrit/nuad090

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Published

30-11-2025

How to Cite

Halim, R., Suzan, R., Hanina, Dewi, H., Ekawaty, F., Hafizah, & Hardiningsih, D. T. (2025). Phytochemical screening of alkaloid compounds on microencapsulation of Moringa oleifera leaf extract. Proceedings Academic Universitas Jambi, 1(2), 716-721. https://doi.org/10.22437/proca.v1i2.50324