Test of Teratogenic Effects of Transflutrin and D-Alletrin Compounds in Mosquito Drugs against Mice Fetus (Mus Musculus L.)

Abstract

Transflutrin and d-alletrin compounds in insect repellents that are widely used by people are classified as insecticides which can be teratogenic. This study aims to determine the teratogenic effects caused by a combination of transflutrin and d-alletrin compounds on fetus mice (Mus musculus L.) during the period of organogenesis. The design used was a randomized block design (RBD) with 4 treatments and 5 groups as replications. The treatment consisted of exposure to a combination of transflutrin and d-alletrin compounds with each dose of 4 mg and 45 mg for 0, 6, 8 and 10 hours at gestational age 6 to 15 days. On the 18th day the pregnancy was carried out laparotomy to take the fetus from the mother's uterus to the mice. The design used was a randomized block design (RBD) with 4 treatments and 5 groups as replications. The treatment consisted of exposure to a combination of transflutrin and d-alletrin compounds with each dose of 4 mg and 45 mg for 0, 6, 8 and 10 hours at gestational age 6 to 15 days. On the 18th day the pregnancy was carried out laparotomy to take the fetus from the mother's uterus to the mice. The results showed that the exposure of 6, 8 and 10 hours of the combination of transflutrin and d-alletrin compounds could cause 33.3%, 40.9% and 38.9% of fetal deaths, 19.1% of hemorrhages, respectively. 36.8% and 50%, body cancer as much as 2.38%, 2.6% and 5.6%, body humping (flexion) by 0%, 5.2% and 11.1%, bone deformities as much as 4.7%, 10, 5% and 16.7%, and abnormalities resulting from ossification of fetal mice were 7.1%, 13.2% and 19.4%. But the treatment of exposure did not affect body weight, body length and fetal morphology of mice. From the results of the study it can be concluded that the combination of transflutrin and d-alletrin compounds have mild teratogenic effects on fetus mice and the teratogenic effects increase with increasing exposure time to external abnormalities and fetal internal mice.